Van den Abbeele et al, 2023, International Journal of Molecular Sciences

After ascertaining the prebiotic potential of tributyrin in a first study, we wanted to discover synbiotic combination further stimulating butyrate production. Relying on the high throughput of the ex vivo SIFR® technology, an exhaustive investigation was set up for the prebiotic (tributyrin) and the probiotics (Lacticaseibacillus rhamnosus GG, Limosilactobacillus reuteri), alone or in combination. To account for inter-individual variations, the gut microbiota of 6 donors was tested in parallel. The experiment confirmed that, while each molecule of tributyrin is easily converted to 3 butyrate on its own, the addition of Lacticaseibacillus rhamnosus GG or Limosilactobacillus reuteri opened an additional pathway of conversion through butyrate by first converting tributyrin-derived glycerol to lactate, which could in turn could be metabolised to butyrate by the endemic microorganism Coprococcus catus.

Van den Abbeele et al, 2023, Microorganisms.

Various bovine plasma fractions were investigated for their prebiotic potential and mechanism of action. To ensure a biorelevant research, the colonic SIFR simulation was preceded by a digestion and absorption step. The immunoglobulin fraction exhibited the most pronounced prebiotic potential. It was used specifically by Bacteroides vulgatus, Lachnoclostridium edouardi and Coprococcus comes, leading to the production of health-promoting SCFA (acetate, propionate & butyrate).